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The issue of determination of tin in environmental matrices
Bušinová, Markéta ; Sommer, Lumír (referee) ; Doležalová Weissmannová, Helena (advisor)
This diploma thesis deals with the retrieval of the content of tin in environmental media, the circulation in them and possibilities of the determination of tin in the environment. Summarizes available information on the properties of tin and its compounds, the occurrence and circulation of tin in environmental and determination of tin in environmental media, attention is paid to the determination of total tin content using method of ET-AAS. The experimental part is focused on optimization methods of determination for the ET AAS and selection of a suitable extraction procedure for determination of total tin content in soils and sediments. The best procedure for the determination of tin in the ET-AAS was applied to samples of soil, sediment and water. As the optimum matrix modifier was chosen the solution of Pd (NO3)2 in 5% ascorbic acid, the optimum pyrolysis temperature was 1400 °C and atomization temperature 2400 ° C. Neither of extraction procedures examined were found to be an appropriate procedure for the determination of tin in environmental samples and further work is necessary to optimize the method of separation.
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Spectrophotometric determination of Chromium(III) with organic reagents
Nevrlá, Jana ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
The theoretical part of this master’s thesis is mainly focused on the physical and chemical properties of chromium, its toxicity and possibility of entering of various forms of chromium into the environment. There are also described the most common analytical methods for determination of chromium in water, including ways of modification and preconcentration water samples. The aim of experimental part is the optimalization and assessing the conditions spectrophotometric determination of Cr(III) by selected organic reagents (chromazurol S, eriochromcyanine R, 4-(2-thiazolylazo)resorcinol) and their comparison with the determination of total chromium, as Cr(III), using atomic absorption spectrometry with electrothermic atomization. The analytical procedure for spectrophotometric determination of chromium with chromazurol S, which was found to be most suitable and ET-AAS, were applied to real water samples.
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The issue of determination of tin in environmental matrices
Bušinová, Markéta ; Sommer, Lumír (referee) ; Doležalová Weissmannová, Helena (advisor)
This diploma thesis deals with the retrieval of the content of tin in environmental media, the circulation in them and possibilities of the determination of tin in the environment. Summarizes available information on the properties of tin and its compounds, the occurrence and circulation of tin in environmental and determination of tin in environmental media, attention is paid to the determination of total tin content using method of ET-AAS. The experimental part is focused on optimization methods of determination for the ET AAS and selection of a suitable extraction procedure for determination of total tin content in soils and sediments. The best procedure for the determination of tin in the ET-AAS was applied to samples of soil, sediment and water. As the optimum matrix modifier was chosen the solution of Pd (NO3)2 in 5% ascorbic acid, the optimum pyrolysis temperature was 1400 °C and atomization temperature 2400 ° C. Neither of extraction procedures examined were found to be an appropriate procedure for the determination of tin in environmental samples and further work is necessary to optimize the method of separation.
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Spectrophotometric determination of Chromium(III) with organic reagents
Nevrlá, Jana ; Komendová, Renata (referee) ; Sommer, Lumír (advisor)
The theoretical part of this master’s thesis is mainly focused on the physical and chemical properties of chromium, its toxicity and possibility of entering of various forms of chromium into the environment. There are also described the most common analytical methods for determination of chromium in water, including ways of modification and preconcentration water samples. The aim of experimental part is the optimalization and assessing the conditions spectrophotometric determination of Cr(III) by selected organic reagents (chromazurol S, eriochromcyanine R, 4-(2-thiazolylazo)resorcinol) and their comparison with the determination of total chromium, as Cr(III), using atomic absorption spectrometry with electrothermic atomization. The analytical procedure for spectrophotometric determination of chromium with chromazurol S, which was found to be most suitable and ET-AAS, were applied to real water samples.
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The application off molecular biological methods in diagnosing the cythomegalovirus in the clinical matherials
ŠIMEROVÁ, Erika
Human cytomegalovirus (HCMV or CMV) is a human viral pathogen from a group of herpetic viruses. Isolation of DNA can be done manually or with the automatic machines - isolators. In my work I concentrated to compare these two different isolations. I did manual isolation with the help of impervious QIAmp DNA Mini Kitu made by QIAGEN and automatic isolation with isolator MagCore using MagCore Genomic whole Blood Kit (RBCBioscience). To compare the isolations blood and plasma were used as clinical materials and their origin was the same. Both methods were used afterwards to set up the qualitative and comparative CMV DNA. The result means that both isolations are comparable - both can be used with the same quantitative result. In this work there is also compared the qualitative determination DNA CMV by using two PCR methods. One of them was the "In house" method in which are two sections of viral DNA CMV ampificated by the couples of specific primers and later there are detected by the agarose gel. Visualisation of these products on agarose gel is going on with the help of intercalatial reagent - ethidium bromide. This method is very sensible but it takes a lot of time.
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